But we must subtract or eliminate sequences where **identical nucleotides are adjacent**, particularly the two A’s and the three C’s or three G’s.

Sun, 03 May 2026 09:22:56 +0000

Optimizing DNA Sequence Quality: The Critical Role of Eliminating Identical Adjacent Nucleotides In bioinformatics and genetic research, maintaining high-quality nucleotide sequences is essential for accurate analysis–from variant calling to evolutionary studies. A particularly important but often overlooked step is the identification and removal of sequences where identical nucleotides are adjacent—specifically, repeated AA, CCC, and GGG trinucleotide runs. These repetitive sequences can severely compromise downstream results and obscure biologically meaningful signals. ### Why Subtract or Eliminate Adjacent Identical Nucleotides? When two or more identical nucleotides occur in sequence—such as AA, CCC, or GGG—they introduce multiple risks in genomic data processing: